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HSPC150/UBE2T Recombinant Antibody, Cy3 Conjugated

Applications

  • WB

Reactivity

  • Human
Overview
Catalog # bsm-62809r-cy3
Product Name HSPC150/UBE2T Recombinant Antibody, Cy3 Conjugated
Applications
WB
Reactivity Human
Specifications
Conjugation Cy3
Host Rabbit
Source KLH conjugated synthetic peptide derived from human UBE2T
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 29089
Swiss Prot Q9NPD8
Subcellular location Cytoplasm, Nucleus
Synonyms HSPC150; PIG50; Ube2t.
Background Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes monoubiquitination. Involved in mitomycin-C (MMC)-induced DNA repair. Acts as a specific E2 ubiquitin-conjugating enzyme for the Fanconi anemia complex by associating with E3 ubiquitin-protein ligase FANCL and catalyzing monoubiquitination of FANCD2, a key step in the DNA damage pathway.
Application Dilution
WB 1:300-5000

Frequently asked questions

Which applications is HSPC150/UBE2T Recombinant Antibody, Cy3 Conjugated (bsm-62809R-Cy3) validated for, and at what dilution?

bsm-62809R-Cy3 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does HSPC150/UBE2T Recombinant Antibody, Cy3 Conjugated (bsm-62809R-Cy3) react with?

Tested reactivity: Human.

How should bsm-62809R-Cy3 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise HSPC150/UBE2T Recombinant Antibody, Cy3 Conjugated (bsm-62809R-Cy3)?

KLH conjugated synthetic peptide derived from human UBE2T

What are the excitation and emission wavelengths of the Cy3 conjugate (bsm-62809R-Cy3)?

Cy3 on bsm-62809R-Cy3: excitation / emission 512,550nm/570,615nm.