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Phospho-SHIP1 (Tyr1022) Recombinant Antibody, PE-Cy5 Conjugated

Applications

  • WB

Reactivity

  • Human
Overview
Catalog # bsm-62834r-pe-cy5
Product Name Phospho-SHIP1 (Tyr1022) Recombinant Antibody, PE-Cy5 Conjugated
Applications
WB
Reactivity Human
Specifications
Conjugation PE-Cy5
Host Rabbit
Source KLH conjugated synthetic peptide derived from human INPP5D around the phosphorylation site of Y1022
Modification Site Tyr1022
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 3635
Swiss Prot Q92835
Subcellular location Cytoplasm, Cell membrane
Synonyms Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1; Inositol polyphosphate-5-phosphatase of 145 kDa; SIP-145; SH2 domain-containing inositol 5'-phosphatase 1; SHIP-1; p150Ship; hp51CN; INPP5D; SHIP.
Background Phosphatidylinositol (PtdIns) phosphatase that specifically hydrolyzes the 5-phosphate of phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3) to produce PtdIns(3,4)P2, thereby negatively regulating the PI3K (phosphoinositide 3-kinase) pathways.
Application Dilution
WB 1:300-5000

Frequently asked questions

Which applications is Phospho-SHIP1 (Tyr1022) Recombinant Antibody, PE-Cy5 Conjugated (bsm-62834R-PE-Cy5) validated for, and at what dilution?

bsm-62834R-PE-Cy5 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Phospho-SHIP1 (Tyr1022) Recombinant Antibody, PE-Cy5 Conjugated (bsm-62834R-PE-Cy5) react with?

Tested reactivity: Human.

How should bsm-62834R-PE-Cy5 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Phospho-SHIP1 (Tyr1022) Recombinant Antibody, PE-Cy5 Conjugated (bsm-62834R-PE-Cy5)?

KLH conjugated synthetic peptide derived from human INPP5D around the phosphorylation site of Y1022

What are the excitation and emission wavelengths of the PE-Cy5 conjugate (bsm-62834R-PE-Cy5)?

PE-Cy5 on bsm-62834R-PE-Cy5: excitation / emission 488nm/670nm.