ISG15 Recombinant Antibody

$Hela (H) cells were treated with or without IFN (1000 U\/mL) for 24 h, 25 \u03bcg total protein per lane of cell lysates (see on figure) probed with ISG15 monoclonal antibody, unconjugated (bsm-62839R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

Hela (H) cells were treated with or without IFN (1000 U\/mL) for 24 h, 25 \u03bcg total protein per lane of cell lysates (see on figure) probed with ISG15 monoclonal antibody, unconjugated (bsm-62839R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

Reactivity

Human

Overview
Catalog #	bsm-62839r
Product Name	ISG15 Recombinant Antibody
Applications	WB
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human ISG15
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified Polyclonal A
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	9636
Swiss Prot	P05161
Synonyms	IP17; Ubiquitin cross-reactive protein; hUCRP; ISG15; G1P2.
Background	Ubiquitin-like protein which plays a key role in the innate immune response to viral infection either via its conjugation to a target protein (ISGylation) or via its action as a free or unconjugated protein. ISGylation involves a cascade of enzymatic reactions involving E1, E2, and E3 enzymes which catalyze the conjugation of ISG15 to a lysine residue in the target protein.
Application Dilution
WB	1:300-5000