Islet-1 Recombinant Antibody, AbBy Fluor-555 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-62842r-bf555 |
| Product Name | Islet-1 Recombinant Antibody, AbBy Fluor-555 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-555 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Islet 1 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3670 |
| Swiss Prot | P61371 |
| Subcellular location | Nucleus |
| Synonyms | Isl-1; ISLET1; Islet1; ISL 1. |
| Background | ISL1 (ISL1 transcription factor, LIM/homeodomain) is a member of the LIM/homeodomain family of transcription factors. It binds to the enhancer region of the insulin gene, among others, and may play an important role in regulating insulin gene expression. It is central to the development of pancreatic cell lineages and may also be required for motor neuron generation. Islet-1 expression defines cardiac progenitor cell populations and is required for normal cardiac development and asymmetry. |
| Application Dilution | |
| WB | 1:500-2000 |
| IF(IHC-P) | IFIHC-P1:100-500 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is Islet-1 Recombinant Antibody, AbBy Fluor-555 Conjugated (bsm-62842R-BF555) validated for, and at what dilution?
bsm-62842R-BF555 is validated for WB(WB) (1:500-2000); IF(IHC-P)(IF(IHC-P)) (1:100-500); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Islet-1 Recombinant Antibody, AbBy Fluor-555 Conjugated (bsm-62842R-BF555) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-62842R-BF555 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Islet-1 Recombinant Antibody, AbBy Fluor-555 Conjugated (bsm-62842R-BF555)?
KLH conjugated synthetic peptide derived from human Islet 1
What are the excitation and emission wavelengths of the AbBy Fluor-555 conjugate (bsm-62842R-BF555)?
AbBy Fluor-555 on bsm-62842R-BF555: excitation / emission 553nm/568nm.