Islet-1 Recombinant Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-62843r |
| Product Name | Islet-1 Recombinant Antibody |
| Applications |
WB
IHC-P
IHC-F
IP
IF
FLOW-CYT
ICC/IF
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Islet 1 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 3670 |
| Swiss Prot | P61371 |
| Subcellular location | Nucleus |
| Synonyms | Isl-1; ISLET1; Islet1; ISL 1; Insulin related protein. |
| Background | ISL1 (ISL1 transcription factor, LIM/homeodomain) is a member of the LIM/homeodomain family of transcription factors. It binds to the enhancer region of the insulin gene, among others, and may play an important role in regulating insulin gene expression. It is central to the development of pancreatic cell lineages and may also be required for motor neuron generation. Islet-1 expression defines cardiac progenitor cell populations and is required for normal cardiac development and asymmetry. |
| Application Dilution | |
| WB | 1:300-5000 |
| IHC-P | FCM1:20-100 |
| IHC-F | IHC-P1:200-400 |
| IP | IHC-F1:100-500 |
| IF | ICC1:50-200 |
| Flow-Cyt | IP1-2ug |
| ICC/IF | IF |
Frequently asked questions
Which applications is Islet-1 Recombinant Antibody (bsm-62843R) validated for, and at what dilution?
bsm-62843R is validated for WB (1:300-5000); FCM (1:20-100); IHC-P (1:200-400); IHC-F (1:100-500); IF(ICC) (1:50-200); IP (1-2ug); IF. These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Islet-1 Recombinant Antibody (bsm-62843R) react with?
Tested reactivity: Human.
How should bsm-62843R be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
What immunogen was used to raise Islet-1 Recombinant Antibody (bsm-62843R)?
KLH conjugated synthetic peptide derived from human Islet 1