ITCH/AIP4 Recombinant Antibody, RBITC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-62844r-rbitc |
| Product Name | ITCH/AIP4 Recombinant Antibody, RBITC Conjugated |
| Applications |
FCM
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human ITCH |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 83737 |
| Swiss Prot | Q96J02 |
| Subcellular location | Cytoplasm, Nucleus, Cell membrane |
| Synonyms | ITCH; AIF4; AIP4; NAPP1. |
| Background | Acts as an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. |
| Application Dilution | |
| FCM | 1:50-100 |
| IF(ICC) | IFICC1:100-500 |
Frequently asked questions
Which applications is ITCH/AIP4 Recombinant Antibody, RBITC Conjugated (bsm-62844R-RBITC) validated for, and at what dilution?
bsm-62844R-RBITC is validated for FCM(FCM) (1:50-100); IF(ICC)(IF(ICC)) (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does ITCH/AIP4 Recombinant Antibody, RBITC Conjugated (bsm-62844R-RBITC) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-62844R-RBITC be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ITCH/AIP4 Recombinant Antibody, RBITC Conjugated (bsm-62844R-RBITC)?
KLH conjugated synthetic peptide derived from human ITCH
What are the excitation and emission wavelengths of the RBITC conjugate (bsm-62844R-RBITC)?
RBITC on bsm-62844R-RBITC: excitation / emission 570nm/595nm.