Phospho-Syk(Tyr323) Recombinant Antibody, Cy5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-62848r-cy5 |
| Product Name | Phospho-Syk(Tyr323) Recombinant Antibody, Cy5 Conjugated |
| Applications |
WB
IF
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Syk around the phosphorylation site of Y323 |
| Modification Site | Tyr323 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 6850 |
| Swiss Prot | P43405 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | KSYK; p72syk; Spleen tyrosine kinase; Syk. |
| Background | Non-receptor tyrosine kinase which mediates signal transduction downstream of a variety of transmembrane receptors including classical immunoreceptors like the B-cell receptor (BCR). Regulates several biological processes including innate and adaptive immunity, cell adhesion, osteoclast maturation, platelet activation and vascular development.Assembles into signaling complexes with activated receptors at the plasma membrane via interaction between its SH2 domains and the receptor tyrosine-phosphorylated ITAM domains. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF | |
Frequently asked questions
Which applications is Phospho-Syk(Tyr323) Recombinant Antibody, Cy5 Conjugated (bsm-62848R-Cy5) validated for, and at what dilution?
bsm-62848R-Cy5 is validated for WB (1:300-5000); IF. These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-Syk(Tyr323) Recombinant Antibody, Cy5 Conjugated (bsm-62848R-Cy5) react with?
Tested reactivity: Human.
How should bsm-62848R-Cy5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-Syk(Tyr323) Recombinant Antibody, Cy5 Conjugated (bsm-62848R-Cy5)?
KLH conjugated synthetic peptide derived from human Syk around the phosphorylation site of Y323
What are the excitation and emission wavelengths of the Cy5 conjugate (bsm-62848R-Cy5)?
Cy5 on bsm-62848R-Cy5: excitation / emission 625,650nm/670nm.