Phospho-eIF4EBP1/2/3(Thr46/Thr46/Thr32) Recombinant Antibody, Cy5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-62853r-cy5 |
| Product Name | Phospho-eIF4EBP1/2/3(Thr46/Thr46/Thr32) Recombinant Antibody, Cy5 Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Human, Mouse |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human 4E BP1 around the phosphorylation site of T46 |
| Modification Site | Thr46/Thr46/Thr32 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1978 |
| Swiss Prot | Q13541, Q13542, O60516 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | 4EBP1; 4EBP2; 4EBP3; eIF4E binding protein 1; eIF4E binding protein 2; eIF4E binding protein 3; PHAS1. |
| Background | eIF4EBP1 is a member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. |
| Application Dilution | |
| WB | 1:500-2000 |
| IF(IHC-P) | IFIHC-P1:50-200 |
Frequently asked questions
Which applications is Phospho-eIF4EBP1/2/3(Thr46/Thr46/Thr32) Recombinant Antibody, Cy5 Conjugated (bsm-62853R-Cy5) validated for, and at what dilution?
bsm-62853R-Cy5 is validated for WB(WB) (1:500-2000); IF(IHC-P)(IF(IHC-P)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-eIF4EBP1/2/3(Thr46/Thr46/Thr32) Recombinant Antibody, Cy5 Conjugated (bsm-62853R-Cy5) react with?
Tested reactivity: Human, Mouse.
How should bsm-62853R-Cy5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-eIF4EBP1/2/3(Thr46/Thr46/Thr32) Recombinant Antibody, Cy5 Conjugated (bsm-62853R-Cy5)?
KLH conjugated synthetic peptide derived from human 4E BP1 around the phosphorylation site of T46
What are the excitation and emission wavelengths of the Cy5 conjugate (bsm-62853R-Cy5)?
Cy5 on bsm-62853R-Cy5: excitation / emission 625,650nm/670nm.