Phospho-PKA C alpha/beta/gamma (T197) Recombinant Antibody, APC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-62860r-apc |
| Product Name | Phospho-PKA C alpha/beta/gamma (T197) Recombinant Antibody, APC Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | APC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human PKA C alpha around the phosphorylation site of T197 |
| Modification Site | Thr197 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5568 |
| Swiss Prot | P22612,P17612,P22694 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | cAMP dependent protein kinase catalytic subunit alpha; cAMP dependent protein kinase catalytic subunit beta; cAMP dependent protein kinase catalytic subunit gamma; PKA C alpha; PKA C beta; PKA C gamma; PKACA; PRKACA; PRKACB; PRKACG. |
| Background | Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis. |
| Application Dilution | |
| WB | 1:500-2000 |
| IF(IHC-P) | IFIHC-P1:50-200 |
Frequently asked questions
Which applications is Phospho-PKA C alpha/beta/gamma (T197) Recombinant Antibody, APC Conjugated (bsm-62860R-APC) validated for, and at what dilution?
bsm-62860R-APC is validated for WB(WB) (1:500-2000); IF(IHC-P)(IF(IHC-P)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-PKA C alpha/beta/gamma (T197) Recombinant Antibody, APC Conjugated (bsm-62860R-APC) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-62860R-APC be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-PKA C alpha/beta/gamma (T197) Recombinant Antibody, APC Conjugated (bsm-62860R-APC)?
KLH conjugated synthetic peptide derived from human PKA C alpha around the phosphorylation site of T197
What are the excitation and emission wavelengths of the APC conjugate (bsm-62860R-APC)?
APC on bsm-62860R-APC: excitation / emission 650nm/660nm.