Phospho-GLUT4 (Ser488) Recombinant Antibody, PE-Cy5.5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-62861r-pe-Cy5.5 |
| Product Name | Phospho-GLUT4 (Ser488) Recombinant Antibody, PE-Cy5.5 Conjugated |
| Applications |
WB
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | PE-Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human GLUT4 around the phosphorylation site of S488 |
| Modification Site | Ser488 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 6517 |
| Swiss Prot | P14672 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | insulin-responsive; Glucose transporter GLUT 4; Glucose transporter type 4; Glucose transporter type 4 insulin responsive; GLUT4; Insulin responsive glucose transporter type 4; SLC2A4 ; Solute carrier family 2 member 4. |
| Background | Insulin-regulated facilitative glucose transporter, which plays a key role in removal of glucose from circulation. Response to insulin is regulated by its intracellular localization: in the absence of insulin, it is efficiently retained intracellularly within storage compartments in muscle and fat cells. Upon insulin stimulation, translocates from these compartments to the cell surface where it transports glucose from the extracellular milieu into the cell. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is Phospho-GLUT4 (Ser488) Recombinant Antibody, PE-Cy5.5 Conjugated (BSM-62861R-PE-CY5-5) validated for, and at what dilution?
BSM-62861R-PE-CY5-5 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-GLUT4 (Ser488) Recombinant Antibody, PE-Cy5.5 Conjugated (BSM-62861R-PE-CY5-5) react with?
Tested reactivity: Human, Mouse, Rat.
How should BSM-62861R-PE-CY5-5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-GLUT4 (Ser488) Recombinant Antibody, PE-Cy5.5 Conjugated (BSM-62861R-PE-CY5-5)?
KLH conjugated synthetic peptide derived from human GLUT4 around the phosphorylation site of S488
What are the excitation and emission wavelengths of the PE-Cy5.5 conjugate (BSM-62861R-PE-CY5-5)?
PE-Cy5.5 on BSM-62861R-PE-CY5-5: excitation / emission 488nm/694nm.