Osteocalcin Recombinant Antibody

$Paraformaldehyde-fixed, paraffin embedded Human osteosarcoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Osteocalcin Monoclonal Antibody, Unconjugated (bsm-62874R) at 1:300 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human osteosarcoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Osteocalcin Monoclonal Antibody, Unconjugated (bsm-62874R) at 1:300 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Applications

Reactivity

Human

Predicted Reactivity

Mouse
Rat

Overview
Catalog #	bsm-62874r
Product Name	Osteocalcin Recombinant Antibody
Applications	WB
Reactivity	Human
Predicted Reactivity	Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	A synthesized peptide derived from human Osteocalcin
Clonality	Recombinant
Isotype	IgG
Concentration	1mg/ml
Purification	purified by Protein A.
Storage Buffer	10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	632
Swiss Prot	P02818
Subcellular location	Secreted
Synonyms	BGP; OC; OCN; Bglap2; Bgpr; Bgpra; OSTCN_BOVIN; BGLAP; Bone Gla protein (BGP); Gamma-carboxyglutamic acid-containing protein; OSTCN_HUMAN; OSTCN_PIG; OSTCN_RAT; bone gamma-carboxyglutamate protein; bone gamma-carboxyglutamate (gla) protein; osteocalcin; bone gla protein
Background	Bone protein that constitutes 1-2% of the total bone protein, and which acts as a negative regulator of bone formation. Functions to limit bone formation without impairing bone resorption or mineralization (By similarity). It binds strongly to apatite and calcium.
Application Dilution
WB	1:300-5000