DCAF13 Recombinant Antibody

$25 ug total protein per lane of various lysates (see on figure) probed with DCAF13 monoclonal antibody, unconjugated (bsm-62879R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with DCAF13 monoclonal antibody, unconjugated (bsm-62879R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-62879r
Product Name	DCAF13 Recombinant Antibody
Applications	WB IP
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human DCAF13
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	25879
Swiss Prot	Q9NV06
Subcellular location	Nucleus
Synonyms	DCAF13; DDB1 and CUL4 associated factor 13; GM83; HSPC064; WD repeat and SOF domain-containing protein 1; WDSOF1.
Background	Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome.
Application Dilution
WB	1:500-2000
IP	IP1:20-50