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Lin28 Recombinant Antibody, AbBy Fluor-680 Conjugated

Applications

  • WB
  • FCM
  • IF(ICC)

Reactivity

  • Human
Overview
Catalog # bsm-62884r-bf680
Product Name Lin28 Recombinant Antibody, AbBy Fluor-680 Conjugated
Applications
WB FCM IF(ICC)
Reactivity Human
Specifications
Conjugation AbBy Fluor-680
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Lin 28A
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 79727
Swiss Prot Q9H9Z2
Subcellular location Cytoplasm, Nucleus
Synonyms Protein lin-28 homolog A; LIN28A; CSDD1; LIN28; ZCCHC1.
Background Seems to recognize a common structural G-quartet (G4) feature in its miRNA and mRNA targets (Probable). 'Translational enhancer' that drives specific mRNAs to polysomes and increases the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in mRNA stabilization.
Application Dilution
WB 1:500-2000
FCM FCM1:50-100
IF(ICC) IFICC1:50-200

Frequently asked questions

Which applications is Lin28 Recombinant Antibody, AbBy Fluor-680 Conjugated (bsm-62884R-BF680) validated for, and at what dilution?

bsm-62884R-BF680 is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Lin28 Recombinant Antibody, AbBy Fluor-680 Conjugated (bsm-62884R-BF680) react with?

Tested reactivity: Human.

How should bsm-62884R-BF680 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Lin28 Recombinant Antibody, AbBy Fluor-680 Conjugated (bsm-62884R-BF680)?

KLH conjugated synthetic peptide derived from human Lin 28A

What are the excitation and emission wavelengths of the AbBy Fluor-680 conjugate (bsm-62884R-BF680)?

AbBy Fluor-680 on bsm-62884R-BF680: excitation / emission 679nm/702nm.