Lin28 Recombinant Antibody, Cy7 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-62884r-cy7 |
| Product Name | Lin28 Recombinant Antibody, Cy7 Conjugated |
| Applications |
WB
FCM
IF(ICC)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Lin 28A |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 79727 |
| Swiss Prot | Q9H9Z2 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | Protein lin-28 homolog A; LIN28A; CSDD1; LIN28; ZCCHC1. |
| Background | Seems to recognize a common structural G-quartet (G4) feature in its miRNA and mRNA targets (Probable). 'Translational enhancer' that drives specific mRNAs to polysomes and increases the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in mRNA stabilization. |
| Application Dilution | |
| WB | 1:500-2000 |
| FCM | FCM1:50-100 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is Lin28 Recombinant Antibody, Cy7 Conjugated (bsm-62884R-Cy7) validated for, and at what dilution?
bsm-62884R-Cy7 is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Lin28 Recombinant Antibody, Cy7 Conjugated (bsm-62884R-Cy7) react with?
Tested reactivity: Human.
How should bsm-62884R-Cy7 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Lin28 Recombinant Antibody, Cy7 Conjugated (bsm-62884R-Cy7)?
KLH conjugated synthetic peptide derived from human Lin 28A
What are the excitation and emission wavelengths of the Cy7 conjugate (bsm-62884R-Cy7)?
Cy7 on bsm-62884R-Cy7: excitation / emission 743nm/767nm.