LC3A Recombinant Antibody, AbBy Fluor-647 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-62899r-bf647 |
| Product Name | LC3A Recombinant Antibody, AbBy Fluor-647 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(ICC)
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-647 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human LC3A |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | 1 mg/ml |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 84557 |
| Swiss Prot | Q9H492 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | MAP1LC3;LC3;LC3A;MAP1ALC3;MAP1BLC3 ;MAP1LC3A. |
| Background | Ubiquitin-like modifier involved in formation of autophagosomal vacuoles .While LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. |
| Application Dilution | |
| WB | 1:1000-5000 |
| IF(IHC-P) | IFIHC-P1:100-500 |
| IF(ICC) | IFICC1:50-200 |
| IF | |
Frequently asked questions
Which applications is LC3A Recombinant Antibody, AbBy Fluor-647 Conjugated (bsm-62899R-BF647) validated for, and at what dilution?
bsm-62899R-BF647 is validated for WB(WB) (1:1000-5000); IF(IHC-P)(IF(IHC-P)) (1:100-500); IF(ICC)(IF(ICC)) (1:50-200); IF. These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does LC3A Recombinant Antibody, AbBy Fluor-647 Conjugated (bsm-62899R-BF647) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-62899R-BF647 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise LC3A Recombinant Antibody, AbBy Fluor-647 Conjugated (bsm-62899R-BF647)?
KLH conjugated synthetic peptide derived from human LC3A
What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bsm-62899R-BF647)?
AbBy Fluor-647 on bsm-62899R-BF647: excitation / emission 650nm/665nm.