Phospho-MEK1 (Thr292) Recombinant Antibody, AbBy Fluor-555 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-62900r-bf555 |
| Product Name | Phospho-MEK1 (Thr292) Recombinant Antibody, AbBy Fluor-555 Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | AbBy Fluor-555 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human MEK1 around the phosphorylation site of T292 |
| Modification Site | Thr292 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5604 |
| Swiss Prot | Q02750 |
| Synonyms | Dual specificity mitogen-activated protein kinase kinase 1; MAP kinase kinase 1; MAPKK 1; MKK1; ERK activator kinase 1; MAPK/ERK kinase 1; MEK 1; MAP2K1; MEK-1; PRKMK1. |
| Background | Microtubule plus-end-directed kinetochore motor which plays an important role in chromosome congression, microtubule-kinetochore conjugation and spindle assembly checkpoint activation. Drives chromosome congression (alignment of chromosomes at the spindle equator resulting in the formation of the metaphase plate) by mediating the lateral sliding of polar chromosomes along spindle microtubules towards the spindle equator and by aiding the establishment and maintenance of connections between kinetochores and spindle microtubules. |
| Application Dilution | |
| WB | 1:500-2000 |
| IF(IHC-P) | IFIHC-P1:50-200 |
Frequently asked questions
Which applications is Phospho-MEK1 (Thr292) Recombinant Antibody, AbBy Fluor-555 Conjugated (bsm-62900R-BF555) validated for, and at what dilution?
bsm-62900R-BF555 is validated for WB(WB) (1:500-2000); IF(IHC-P)(IF(IHC-P)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-MEK1 (Thr292) Recombinant Antibody, AbBy Fluor-555 Conjugated (bsm-62900R-BF555) react with?
Tested reactivity: Human.
How should bsm-62900R-BF555 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-MEK1 (Thr292) Recombinant Antibody, AbBy Fluor-555 Conjugated (bsm-62900R-BF555)?
KLH conjugated synthetic peptide derived from human MEK1 around the phosphorylation site of T292
What are the excitation and emission wavelengths of the AbBy Fluor-555 conjugate (bsm-62900R-BF555)?
AbBy Fluor-555 on bsm-62900R-BF555: excitation / emission 553nm/568nm.