CD10 Recombinant Antibody, Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-62909r-cy3 |
| Product Name | CD10 Recombinant Antibody, Cy3 Conjugated |
| Applications |
WB
FCM
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Neprilysin |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4311 |
| Swiss Prot | P08473 |
| Subcellular location | Cell membrane |
| Synonyms | Atriopeptidase; CALLA; CD10; CD10 antigen;Enkephalinase; EPN; Membrane metallo endopeptidase (neutral endopeptidase, enkephalinase, CALLA, CD10); CALLA CD10; NEP; Neprilysin; Neutral endopeptidase. |
| Background | Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. |
| Application Dilution | |
| WB | 1:1000-2000 |
| FCM | FCM1ug/Test |
| IF(ICC) | IFICC1:100-200 |
Frequently asked questions
Which applications is CD10 Recombinant Antibody, Cy3 Conjugated (bsm-62909R-Cy3) validated for, and at what dilution?
bsm-62909R-Cy3 is validated for WB(WB) (1:1000-2000); FCM(FCM) (1ug/Test); IF(ICC)(IF(ICC)) (1:100-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CD10 Recombinant Antibody, Cy3 Conjugated (bsm-62909R-Cy3) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-62909R-Cy3 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CD10 Recombinant Antibody, Cy3 Conjugated (bsm-62909R-Cy3)?
KLH conjugated synthetic peptide derived from human Neprilysin
What are the excitation and emission wavelengths of the Cy3 conjugate (bsm-62909R-Cy3)?
Cy3 on bsm-62909R-Cy3: excitation / emission 512,550nm/570,615nm.