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Nucleolin Recombinant Antibody, Biotin Conjugated

Applications

  • IHC-P
  • IHC-F

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-62918r-biotin
Product Name Nucleolin Recombinant Antibody, Biotin Conjugated
Applications
IHC-P IHC-F
Reactivity Human, Mouse, Rat
Specifications
Conjugation Biotin
Host Rabbit
Source KLH conjugated synthetic peptide derived from human NCL
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C for 12 months.
Target
Gene ID 4691
Swiss Prot P19338
Subcellular location Cytoplasm, Nucleus
Synonyms NCL; Nucl; Nucleolin; Protein C23.
Background Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats.
Application Dilution
IHC-P 1:100-500
IHC-F IHC-F1:100-500

Frequently asked questions

Which applications is Nucleolin Recombinant Antibody, Biotin Conjugated (bsm-62918R-Biotin) validated for, and at what dilution?

bsm-62918R-Biotin is validated for IHC-P(IHC-P) (1:100-500); IHC-F(IHC-F) (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Nucleolin Recombinant Antibody, Biotin Conjugated (bsm-62918R-Biotin) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-62918R-Biotin be stored?

Store at -20°C for 12 months. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Nucleolin Recombinant Antibody, Biotin Conjugated (bsm-62918R-Biotin)?

KLH conjugated synthetic peptide derived from human NCL