Nucleolin Recombinant Antibody, FITC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-62918r-fitc |
| Product Name | Nucleolin Recombinant Antibody, FITC Conjugated |
| Applications |
FCM
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | FITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human NCL |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4691 |
| Swiss Prot | P19338 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | NCL; Nucl; Nucleolin; Protein C23. |
| Background | Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats. |
| Application Dilution | |
| FCM | 1:50-100 |
| IF(IHC-P) | IFIHC-P1:100-500 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is Nucleolin Recombinant Antibody, FITC Conjugated (bsm-62918R-FITC) validated for, and at what dilution?
bsm-62918R-FITC is validated for FCM(FCM) (1:50-100); IF(IHC-P)(IF(IHC-P)) (1:100-500); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Nucleolin Recombinant Antibody, FITC Conjugated (bsm-62918R-FITC) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-62918R-FITC be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Nucleolin Recombinant Antibody, FITC Conjugated (bsm-62918R-FITC)?
KLH conjugated synthetic peptide derived from human NCL
What are the excitation and emission wavelengths of the FITC conjugate (bsm-62918R-FITC)?
FITC on bsm-62918R-FITC: excitation / emission 494nm/518nm.