NDUFS4 Recombinant Antibody, PE-Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bsm-62919r-pe-Cy5.5 |
| Product Name | NDUFS4 Recombinant Antibody, PE-Cy5.5 Conjugated |
| Applications |
WB
FCM
|
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human |
| Specifications | |
| Conjugation | PE-Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human NDUFS4 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4724 |
| Swiss Prot | O43181 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | AQDQ; CI 18; CI AQDQ; Complex I 18 kDa; NDUFS4. |
| Background | Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. |
| Application Dilution | |
| WB | 1:500-2000 |
| FCM | FCM1:50-100 |
Frequently asked questions
Which applications is NDUFS4 Recombinant Antibody, PE-Cy5.5 Conjugated (BSM-62919R-PE-CY5-5) validated for, and at what dilution?
BSM-62919R-PE-CY5-5 is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does NDUFS4 Recombinant Antibody, PE-Cy5.5 Conjugated (BSM-62919R-PE-CY5-5) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human.
How should BSM-62919R-PE-CY5-5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise NDUFS4 Recombinant Antibody, PE-Cy5.5 Conjugated (BSM-62919R-PE-CY5-5)?
KLH conjugated synthetic peptide derived from human NDUFS4
What are the excitation and emission wavelengths of the PE-Cy5.5 conjugate (BSM-62919R-PE-CY5-5)?
PE-Cy5.5 on BSM-62919R-PE-CY5-5: excitation / emission 488nm/694nm.