NDUFS4 Recombinant Antibody, RBITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bsm-62919r-rbitc |
| Product Name | NDUFS4 Recombinant Antibody, RBITC Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(ICC)
|
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human NDUFS4 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4724 |
| Swiss Prot | O43181 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | AQDQ; CI 18; CI AQDQ; Complex I 18 kDa; NDUFS4. |
| Background | Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. |
| Application Dilution | |
| WB | 1:500-2000 |
| FCM | FCM1:50-100 |
| IF(IHC-P) | IFIHC-P1:100-500 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is NDUFS4 Recombinant Antibody, RBITC Conjugated (bsm-62919R-RBITC) validated for, and at what dilution?
bsm-62919R-RBITC is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(IHC-P)(IF(IHC-P)) (1:100-500); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does NDUFS4 Recombinant Antibody, RBITC Conjugated (bsm-62919R-RBITC) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human.
How should bsm-62919R-RBITC be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise NDUFS4 Recombinant Antibody, RBITC Conjugated (bsm-62919R-RBITC)?
KLH conjugated synthetic peptide derived from human NDUFS4
What are the excitation and emission wavelengths of the RBITC conjugate (bsm-62919R-RBITC)?
RBITC on bsm-62919R-RBITC: excitation / emission 570nm/595nm.