Ras Recombinant Antibody, APC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-62921r-apc-100uL |
| Product Name | Ras Recombinant Antibody, APC Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | APC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Ras |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | 1mg/ml |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4893 |
| Swiss Prot | P01111,P01112,P01116 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | GTPase HRas; GTPase KRas; GTPase NRas; H-Ras-1; H-RASIDX; Ha-Ras; HAMSV; HRAS; K RAS2A; K RAS2B; K RAS4A; K RAS4B; K-RAS; KRAS; N-RAS; N-terminally processed; NRAS; p21ras; RASH1; RASK2. |
| Background | Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. |
| Application Dilution | |
| WB | 1:500-2000 |
| FCM | FCM1:50-100 |
| IF(IHC-P) | IFIHC-P1:100-500 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is Ras Recombinant Antibody, APC Conjugated (bsm-62921R-APC) validated for, and at what dilution?
bsm-62921R-APC is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(IHC-P)(IF(IHC-P)) (1:100-500); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Ras Recombinant Antibody, APC Conjugated (bsm-62921R-APC) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-62921R-APC be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Ras Recombinant Antibody, APC Conjugated (bsm-62921R-APC)?
KLH conjugated synthetic peptide derived from human Ras
What are the excitation and emission wavelengths of the APC conjugate (bsm-62921R-APC)?
APC on bsm-62921R-APC: excitation / emission 650nm/660nm.