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NRAS Recombinant Antibody, Cy3 Conjugated

Applications

  • WB
  • FCM

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-62922r-cy3-100uL
Product Name NRAS Recombinant Antibody, Cy3 Conjugated
Applications
WB FCM
Reactivity Human, Mouse, Rat
Specifications
Conjugation Cy3
Host Rabbit
Source KLH conjugated synthetic peptide derived from human NRAS
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 4893
Swiss Prot P01111,P01112,P01116
Subcellular location Cytoplasm, Cell membrane
Synonyms GTPase HRas;GTPase KRas;GTPase NRas;H-Ras-1;HAMSV;HRAS;HRAS1;K-RAS;N-RAS;NRAS;p21ras;RASH1;RASK2;Transforming protein p21.
Background Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.
Application Dilution
WB 1:500-2000
FCM FCM1:50-100

Frequently asked questions

Which applications is NRAS Recombinant Antibody, Cy3 Conjugated (bsm-62922R-Cy3) validated for, and at what dilution?

bsm-62922R-Cy3 is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does NRAS Recombinant Antibody, Cy3 Conjugated (bsm-62922R-Cy3) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-62922R-Cy3 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise NRAS Recombinant Antibody, Cy3 Conjugated (bsm-62922R-Cy3)?

KLH conjugated synthetic peptide derived from human NRAS

What are the excitation and emission wavelengths of the Cy3 conjugate (bsm-62922R-Cy3)?

Cy3 on bsm-62922R-Cy3: excitation / emission 512,550nm/570,615nm.