Phospho-PAK1/2/3 (Ser144/Ser141/Ser154) Recombinant Antibody, AbBy Fluor-488 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-62927r-bf488 |
| Product Name | Phospho-PAK1/2/3 (Ser144/Ser141/Ser154) Recombinant Antibody, AbBy Fluor-488 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-488 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human PAK1 around the phosphorylation site of S144 |
| Modification Site | Ser144/Ser141/Ser154 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5058 |
| Swiss Prot | Q13153,Q13177,O75914 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | ADRB2; Alpha-PAK; CDC42/RAC effector kinase PAK-A; EC 2.7.11.1; P65-PAK; P68-PAK; PAK1 (phospho S144); PAK2 (phospho S141); PAK3 (phospho S139). |
| Background | Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, or cell cycle regulation. |
| Application Dilution | |
| WB | 1:500-5000 |
| FCM | FCM1:50-100 |
| IF(IHC-P) | IFIHC-P1:100-200 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is Phospho-PAK1/2/3 (Ser144/Ser141/Ser154) Recombinant Antibody, AbBy Fluor-488 Conjugated (bsm-62927R-BF488) validated for, and at what dilution?
bsm-62927R-BF488 is validated for WB(WB) (1:500-5000); FCM(FCM) (1:50-100); IF(IHC-P)(IF(IHC-P)) (1:100-200); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-PAK1/2/3 (Ser144/Ser141/Ser154) Recombinant Antibody, AbBy Fluor-488 Conjugated (bsm-62927R-BF488) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-62927R-BF488 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-PAK1/2/3 (Ser144/Ser141/Ser154) Recombinant Antibody, AbBy Fluor-488 Conjugated (bsm-62927R-BF488)?
KLH conjugated synthetic peptide derived from human PAK1 around the phosphorylation site of S144
What are the excitation and emission wavelengths of the AbBy Fluor-488 conjugate (bsm-62927R-BF488)?
AbBy Fluor-488 on bsm-62927R-BF488: excitation / emission 499nm/519nm.