PAK1 Recombinant Antibody, AbBy Fluor-680 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-62929r-bf680 |
| Product Name | PAK1 Recombinant Antibody, AbBy Fluor-680 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-680 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human PAK1 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5058 |
| Swiss Prot | Q13153 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | ADRB2; p21 activated kinase 1; p65 PAK; PAK alpha; Pak1; Paka; PAKalpha; Protein kinase MUK2. |
| Background | Protein kinase involved in intracellular signaling pathways downstream of integrins and receptor-type kinases that plays an important role in cytoskeleton dynamics, in cell adhesion, migration, proliferation, apoptosis, mitosis, and in vesicle-mediated transport processes. |
| Application Dilution | |
| WB | 1:500-2000 |
| FCM | FCM1:50-100 |
| IF(IHC-P) | IFIHC-P1:100-500 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is PAK1 Recombinant Antibody, AbBy Fluor-680 Conjugated (bsm-62929R-BF680) validated for, and at what dilution?
bsm-62929R-BF680 is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(IHC-P)(IF(IHC-P)) (1:100-500); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does PAK1 Recombinant Antibody, AbBy Fluor-680 Conjugated (bsm-62929R-BF680) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-62929R-BF680 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PAK1 Recombinant Antibody, AbBy Fluor-680 Conjugated (bsm-62929R-BF680)?
KLH conjugated synthetic peptide derived from human PAK1
What are the excitation and emission wavelengths of the AbBy Fluor-680 conjugate (bsm-62929R-BF680)?
AbBy Fluor-680 on bsm-62929R-BF680: excitation / emission 679nm/702nm.