PIM2 Recombinant Antibody

$25 ug total protein per lane of various lysates (see on figure) probed with PIM2 monoclonal antibody, unconjugated (bsm-62933R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with PIM2 monoclonal antibody, unconjugated (bsm-62933R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

WB
IP
ICC/IF

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-62933r
Product Name	PIM2 Recombinant Antibody
Applications	WB IP ICC/IF
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human PIM2
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	11040
Swiss Prot	Q9P1W9
Synonyms	PIM2; Pim2h.
Background	Proto-oncogene with serine/threonine kinase activity involved in cell survival and cell proliferation. Exerts its oncogenic activity through: the regulation of MYC transcriptional activity, the regulation of cell cycle progression, the regulation of cap-dependent protein translation and through survival signaling by phosphorylation of a pro-apoptotic protein, BAD. Phosphorylation of MYC leads to an increase of MYC protein stability and thereby an increase transcriptional activity.
Application Dilution
WB	1:300-5000
IP	IFICC1:50-200
ICC/IF	IP1-2ug