Phospho-PLCB3 (Ser537) Recombinant Antibody

$The Hela (treated with anisomycin (20\u03bcg\/ml for 15min)) (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-Phospho-PLCB3 (Ser537) antibody (bsm-62936R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.$

The Hela (treated with anisomycin (20\u03bcg\/ml for 15min)) (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-Phospho-PLCB3 (Ser537) antibody (bsm-62936R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.

$4% Paraformaldehyde-fixed Hela (treated with anisomycin (20\u03bcg\/ml for 15min)) (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (Phospho-PLCB3 (Ser537)) monoclonal Antibody, unconjugated (bsm-62936R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.$

4% Paraformaldehyde-fixed Hela (treated with anisomycin (20\u03bcg\/ml for 15min)) (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (Phospho-PLCB3 (Ser537)) monoclonal Antibody, unconjugated (bsm-62936R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

Applications

Reactivity

Human

Overview
Catalog #	bsm-62936r-100ul
Product Name	Phospho-PLCB3 (Ser537) Recombinant Antibody
Applications	WB
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human PLCB3 around the phosphorylation site of S537
Modification Site	Ser537
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	5331
Swiss Prot	Q01970
Subcellular location	Cell membrane
Synonyms	Plcb3.
Background	The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.
Application Dilution
WB	1:500-2000