Phospho-POLR2A (Ser2) Recombinant Antibody, AbBy Fluor-680 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-62939r-bf680-100uL |
| Product Name | Phospho-POLR2A (Ser2) Recombinant Antibody, AbBy Fluor-680 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-680 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human POLR2A around the phosphorylation site of S2 |
| Modification Site | Ser2 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5430 |
| Swiss Prot | P24928 |
| Subcellular location | Nucleus |
| Synonyms | POLR2A; POLR2; RNA polymerase II CTD repeat YSPTSPS. |
| Background | During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing. |
| Application Dilution | |
| WB | 1:500-2000 |
| FCM | FCM1:50-100 |
| IF(IHC-P) | IFIHC-P1:100-500 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is Phospho-POLR2A (Ser2) Recombinant Antibody, AbBy Fluor-680 Conjugated (bsm-62939R-BF680) validated for, and at what dilution?
bsm-62939R-BF680 is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(IHC-P)(IF(IHC-P)) (1:100-500); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-POLR2A (Ser2) Recombinant Antibody, AbBy Fluor-680 Conjugated (bsm-62939R-BF680) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-62939R-BF680 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-POLR2A (Ser2) Recombinant Antibody, AbBy Fluor-680 Conjugated (bsm-62939R-BF680)?
KLH conjugated synthetic peptide derived from human POLR2A around the phosphorylation site of S2
What are the excitation and emission wavelengths of the AbBy Fluor-680 conjugate (bsm-62939R-BF680)?
AbBy Fluor-680 on bsm-62939R-BF680: excitation / emission 679nm/702nm.