Phospho-POLR2A (Ser5) Recombinant Monoclonal Antibody, APC-Cy5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-62940r-apc-cy5-100uL |
| Product Name | Phospho-POLR2A (Ser5) Recombinant Monoclonal Antibody, APC-Cy5 Conjugated |
| Applications |
WB
FCM
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | APC-Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human POLR2A around the phosphorylation site of S5 |
| Modification Site | Ser5 |
| Clonality | Monoclonal |
| Clone # | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5430 |
| Swiss Prot | P24928 |
| Subcellular location | Nucleus |
| Synonyms | POLR2A; POLR2; RNA polymerase II CTD repeat YSPTSPS. |
| Background | During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Phospho-POLR2A (Ser5) Recombinant Monoclonal Antibody, APC-Cy5 Conjugated (bsm-62940R-APC-Cy5) validated for, and at what dilution?
bsm-62940R-APC-Cy5 is validated for WB (1:300-5000); FCM (1:20-100); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-POLR2A (Ser5) Recombinant Monoclonal Antibody, APC-Cy5 Conjugated (bsm-62940R-APC-Cy5) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-62940R-APC-Cy5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-POLR2A (Ser5) Recombinant Monoclonal Antibody, APC-Cy5 Conjugated (bsm-62940R-APC-Cy5)?
KLH conjugated synthetic peptide derived from human POLR2A around the phosphorylation site of S5
What are the excitation and emission wavelengths of the APC-Cy5 conjugate (bsm-62940R-APC-Cy5)?
APC-Cy5 on bsm-62940R-APC-Cy5: excitation / emission 650nm/670nm.