PKC beta Recombinant Monoclonal Antibody, PE-Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-62951r-pe-cy3-100uL |
| Product Name | PKC beta Recombinant Monoclonal Antibody, PE-Cy3 Conjugated |
| Applications |
WB
FCM
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | PE-Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human PKC beta |
| Clonality | Monoclonal |
| Clone # | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5579 |
| Swiss Prot | P05771 |
| Subcellular location | Cytoplasm, Nucleus, Cell membrane |
| Synonyms | protein kinase C beta typeŁťPRKCBŁťPrkcbŁťPKC-B; PKC-betaŁťPRKCB2ŁťPKC Beta-II. |
| Background | Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase involved in various cellular processes such as regulation of the B-cell receptor (BCR) signalosome, oxidative stress-induced apoptosis, androgen receptor-dependent transcription regulation, insulin signaling and endothelial cells proliferation. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is PKC beta Recombinant Monoclonal Antibody, PE-Cy3 Conjugated (bsm-62951R-PE-Cy3) validated for, and at what dilution?
bsm-62951R-PE-Cy3 is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does PKC beta Recombinant Monoclonal Antibody, PE-Cy3 Conjugated (bsm-62951R-PE-Cy3) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-62951R-PE-Cy3 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PKC beta Recombinant Monoclonal Antibody, PE-Cy3 Conjugated (bsm-62951R-PE-Cy3)?
KLH conjugated synthetic peptide derived from human PKC beta
What are the excitation and emission wavelengths of the PE-Cy3 conjugate (bsm-62951R-PE-Cy3)?
PE-Cy3 on bsm-62951R-PE-Cy3: excitation / emission 488nmn/578nm.