PKC delta/theta Recombinant Antibody, PE Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-62953r-pe-100uL |
| Product Name | PKC delta/theta Recombinant Antibody, PE Conjugated |
| Applications |
WB
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | PE |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human PKC delta |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5580 |
| Swiss Prot | Q05655,Q04759,P28867 |
| Subcellular location | Cytoplasm, Nucleus, Cell membrane |
| Synonyms | ALPS3; CVID9; MAY1; nPKC-delta; nPKC-theta; PKCD; PRKCD; PRKCQ; PRKCT; Protein kinase C delta type; Protein kinase C theta type; SDK1; Sphingosine-dependent protein kinase-1; Tyrosine-protein kinase PRKCD. |
| Background | Calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that plays contrasting roles in cell death and cell survival by functioning as a pro-apoptotic protein during DNA damage-induced apoptosis, but acting as an anti-apoptotic protein during cytokine receptor-initiated cell death, is involved in tumor suppression as well as survival of several cancers, is required for oxygen radical production by NADPH oxidase and acts as positive or negative regulator in platelet functional responses. |
| Application Dilution | |
| WB | 1:1000-5000 |
| IF(IHC-P) | IFIHC-P1:100-500 |
| IF(ICC) | IFICC1:100-500 |
Frequently asked questions
Which applications is PKC delta/theta Recombinant Antibody, PE Conjugated (bsm-62953R-PE) validated for, and at what dilution?
bsm-62953R-PE is validated for WB(WB) (1:1000-5000); IF(IHC-P)(IF(IHC-P)) (1:100-500); IF(ICC)(IF(ICC)) (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does PKC delta/theta Recombinant Antibody, PE Conjugated (bsm-62953R-PE) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-62953R-PE be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PKC delta/theta Recombinant Antibody, PE Conjugated (bsm-62953R-PE)?
KLH conjugated synthetic peptide derived from human PKC delta
What are the excitation and emission wavelengths of the PE conjugate (bsm-62953R-PE)?
PE on bsm-62953R-PE: excitation / emission 496,564nm/578nm.