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Phospho-PKC delta (Thr507) Recombinant Antibody, Cy3 Conjugated

Applications

  • WB

Reactivity

  • Human
Overview
Catalog # bsm-62954r-cy3-100uL
Product Name Phospho-PKC delta (Thr507) Recombinant Antibody, Cy3 Conjugated
Applications
WB
Reactivity Human
Specifications
Conjugation Cy3
Host Rabbit
Source KLH conjugated synthetic peptide derived from human PKC delta around the phosphorylation site of T507
Modification Site Thr507
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 5580
Swiss Prot Q05655
Subcellular location Cytoplasm, Nucleus, Cell membrane
Synonyms CVID9; KPCD; MAY1; nPKC delta; PCKd; PKC delta; PKCD; PKCdelta; PRKC D; PRKC delta; Prkcd.
Background Calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that plays contrasting roles in cell death and cell survival by functioning as a pro-apoptotic protein during DNA damage-induced apoptosis, but acting as an anti-apoptotic protein during cytokine receptor-initiated cell death, is involved in tumor suppression as well as survival of several cancers, is required for oxygen radical production by NADPH oxidase and acts as positive or negative regulator in platelet functional responses.
Application Dilution
WB 1:300-5000

Frequently asked questions

Which applications is Phospho-PKC delta (Thr507) Recombinant Antibody, Cy3 Conjugated (bsm-62954R-Cy3) validated for, and at what dilution?

bsm-62954R-Cy3 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Phospho-PKC delta (Thr507) Recombinant Antibody, Cy3 Conjugated (bsm-62954R-Cy3) react with?

Tested reactivity: Human.

How should bsm-62954R-Cy3 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Phospho-PKC delta (Thr507) Recombinant Antibody, Cy3 Conjugated (bsm-62954R-Cy3)?

KLH conjugated synthetic peptide derived from human PKC delta around the phosphorylation site of T507

What are the excitation and emission wavelengths of the Cy3 conjugate (bsm-62954R-Cy3)?

Cy3 on bsm-62954R-Cy3: excitation / emission 512,550nm/570,615nm.