PKC epsilon Recombinant Antibody, RBITC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-62955r-rbitc-100uL |
| Product Name | PKC epsilon Recombinant Antibody, RBITC Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human PKC epsilon |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5581 |
| Swiss Prot | Q02156 |
| Subcellular location | Cytoplasm, Nucleus, Cell membrane |
| Synonyms | nPKC epsilon; PKCE; Pkcea; PRKCE; Protein kinase C epsilon; Protein kinase C epsilon type. |
| Background | Calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that plays essential roles in the regulation of multiple cellular processes linked to cytoskeletal proteins, such as cell adhesion, motility, migration and cell cycle, functions in neuron growth and ion channel regulation, and is involved in immune response, cancer cell invasion and regulation of apoptosis. |
| Application Dilution | |
| WB | 1:500-2000 |
| FCM | FCM1:50-100 |
| IF(IHC-P) | IFIHC-P1:50-200 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is PKC epsilon Recombinant Antibody, RBITC Conjugated (bsm-62955R-RBITC) validated for, and at what dilution?
bsm-62955R-RBITC is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(IHC-P)(IF(IHC-P)) (1:50-200); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does PKC epsilon Recombinant Antibody, RBITC Conjugated (bsm-62955R-RBITC) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-62955R-RBITC be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PKC epsilon Recombinant Antibody, RBITC Conjugated (bsm-62955R-RBITC)?
KLH conjugated synthetic peptide derived from human PKC epsilon
What are the excitation and emission wavelengths of the RBITC conjugate (bsm-62955R-RBITC)?
RBITC on bsm-62955R-RBITC: excitation / emission 570nm/595nm.