Phospho-PKC epsilon (Ser729) Recombinant Antibody, AbBy Fluor-405 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-62956r-bf405-100uL |
| Product Name | Phospho-PKC epsilon (Ser729) Recombinant Antibody, AbBy Fluor-405 Conjugated |
| Applications |
WB
|
| Reactivity | Human, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-405 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human PKC epsilon around the phosphorylation site of S729 |
| Modification Site | Ser729 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5581 |
| Swiss Prot | Q02156 |
| Subcellular location | Cytoplasm, Nucleus, Cell membrane |
| Synonyms | PKC alpha; PKC beta; PKC delta; PKC epsilon; PKC gamma; PKC zeta; PKC2; PKCA; PKCB; PKCD; PKCE; PKCG; PRKCA; PRKCB; PRKCD; PRKCE; PRKCG; PRKCZ. |
| Background | Calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that plays essential roles in the regulation of multiple cellular processes linked to cytoskeletal proteins, such as cell adhesion, motility, migration and cell cycle, functions in neuron growth and ion channel regulation, and is involved in immune response, cancer cell invasion and regulation of apoptosis. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is Phospho-PKC epsilon (Ser729) Recombinant Antibody, AbBy Fluor-405 Conjugated (bsm-62956R-BF405) validated for, and at what dilution?
bsm-62956R-BF405 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-PKC epsilon (Ser729) Recombinant Antibody, AbBy Fluor-405 Conjugated (bsm-62956R-BF405) react with?
Tested reactivity: Human, Rat.
How should bsm-62956R-BF405 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-PKC epsilon (Ser729) Recombinant Antibody, AbBy Fluor-405 Conjugated (bsm-62956R-BF405)?
KLH conjugated synthetic peptide derived from human PKC epsilon around the phosphorylation site of S729
What are the excitation and emission wavelengths of the AbBy Fluor-405 conjugate (bsm-62956R-BF405)?
AbBy Fluor-405 on bsm-62956R-BF405: excitation / emission 401nm/421nm.