PKC Recombinant Monoclonal Antibody, APC-Cy5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-62958r-apc-cy5-100uL |
| Product Name | PKC Recombinant Monoclonal Antibody, APC-Cy5 Conjugated |
| Applications |
WB
FCM
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | APC-Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human PKC |
| Clonality | Monoclonal |
| Clone # | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5582 |
| Swiss Prot | P05129,P05771, P17252,Q02156,Q05513,Q05655 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | PKC alpha; PKC beta; PKC delta; PKC epsilon; PKC gamma; PKC zeta; PKC-A; PKC-alpha; PKC2; PKCA; PKCB; PKCD; PKCE; PKCG; PRKCA; PRKCB; PRKCB1; PRKCB2; PRKCD; PRKCE; PRKCG; PRKCZ; Protein kinase C alpha; Protein kinase C alpha type; Protein kinase C; Protein kinase C beta; Protein kinase C delta; Protein kinase C epsilon; Protein kinase C gamma; Protein kinase C zeta. |
| Background | Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that plays diverse roles in neuronal cells and eye tissues, such as regulation of the neuronal receptors GRIA4/GLUR4 and GRIN1/NMDAR1, modulation of receptors and neuronal functions related to sensitivity to opiates, pain and alcohol, mediation of synaptic function and cell survival after ischemia, and inhibition of gap junction activity after oxidative stress. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is PKC Recombinant Monoclonal Antibody, APC-Cy5 Conjugated (bsm-62958R-APC-Cy5) validated for, and at what dilution?
bsm-62958R-APC-Cy5 is validated for WB (1:300-5000); FCM (1:20-100); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does PKC Recombinant Monoclonal Antibody, APC-Cy5 Conjugated (bsm-62958R-APC-Cy5) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-62958R-APC-Cy5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PKC Recombinant Monoclonal Antibody, APC-Cy5 Conjugated (bsm-62958R-APC-Cy5)?
KLH conjugated synthetic peptide derived from human PKC
What are the excitation and emission wavelengths of the APC-Cy5 conjugate (bsm-62958R-APC-Cy5)?
APC-Cy5 on bsm-62958R-APC-Cy5: excitation / emission 650nm/670nm.