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Phospho-RPS6KB1 (Thr421/Ser424) Recombinant Antibody, AbBy Fluor-405 Conjugated

Applications

  • WB

Reactivity

  • Human
  • Rat
Overview
Catalog # bsm-62970r-bf405-100uL
Product Name Phospho-RPS6KB1 (Thr421/Ser424) Recombinant Antibody, AbBy Fluor-405 Conjugated
Applications
WB
Reactivity Human, Rat
Specifications
Conjugation AbBy Fluor-405
Host Rabbit
Source KLH conjugated synthetic peptide derived from human p70 S6 Kinase around the phosphorylation site of T421/S424
Modification Site Thr421/Ser424
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 6198
Swiss Prot P23443
Subcellular location Cytoplasm, Nucleus
Synonyms EC 2.7.11.1, KS6B1, P70-S6K, RPS6KB1, Ribosomal protein S6 kinase, Ribosomal protein S6 kinase, 70kDa, polypeptide 1, S6K, kinase p70S6K, p70-S6K.
Background Acts to integrate nutrient and growth factor signals in regulation of protein synthesis, cell proliferation, cell growth, cell cycle progression and cell survival. Downstream effector of the mTOR signaling pathway.
Application Dilution
WB 1:500-2000

Frequently asked questions

Which applications is Phospho-RPS6KB1 (Thr421/Ser424) Recombinant Antibody, AbBy Fluor-405 Conjugated (bsm-62970R-BF405) validated for, and at what dilution?

bsm-62970R-BF405 is validated for WB(WB) (1:500-2000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Phospho-RPS6KB1 (Thr421/Ser424) Recombinant Antibody, AbBy Fluor-405 Conjugated (bsm-62970R-BF405) react with?

Tested reactivity: Human, Rat.

How should bsm-62970R-BF405 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Phospho-RPS6KB1 (Thr421/Ser424) Recombinant Antibody, AbBy Fluor-405 Conjugated (bsm-62970R-BF405)?

KLH conjugated synthetic peptide derived from human p70 S6 Kinase around the phosphorylation site of T421/S424

What are the excitation and emission wavelengths of the AbBy Fluor-405 conjugate (bsm-62970R-BF405)?

AbBy Fluor-405 on bsm-62970R-BF405: excitation / emission 401nm/421nm.