EDG3 Recombinant Antibody, Cy5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-62975r-cy5-100uL |
| Product Name | EDG3 Recombinant Antibody, Cy5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human EDG3 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1903 |
| Swiss Prot | Q99500 |
| Subcellular location | Cell membrane |
| Synonyms | EDG3; LPB3; S1P receptor 3; S1P3; S1pr3. |
| Background | Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. When expressed in rat HTC4 hepatoma cells, is capable of mediating S1P-induced cell proliferation and suppression of apoptosis. |
| Application Dilution | |
| WB | 1:1000-2000 |
| IF(IHC-P) | IFIHC-P1:100-200 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is EDG3 Recombinant Antibody, Cy5 Conjugated (bsm-62975R-Cy5) validated for, and at what dilution?
bsm-62975R-Cy5 is validated for WB(WB) (1:1000-2000); IF(IHC-P)(IF(IHC-P)) (1:100-200); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does EDG3 Recombinant Antibody, Cy5 Conjugated (bsm-62975R-Cy5) react with?
Tested reactivity: Human.
How should bsm-62975R-Cy5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise EDG3 Recombinant Antibody, Cy5 Conjugated (bsm-62975R-Cy5)?
KLH conjugated synthetic peptide derived from human EDG3
What are the excitation and emission wavelengths of the Cy5 conjugate (bsm-62975R-Cy5)?
Cy5 on bsm-62975R-Cy5: excitation / emission 625,650nm/670nm.