SATB2 Recombinant Antibody, APC-Cy7 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-62976r-apc-cy7-100uL |
| Product Name | SATB2 Recombinant Antibody, APC-Cy7 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | APC-Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human SATB2 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 23314 |
| Swiss Prot | Q9UPW6 |
| Subcellular location | Nucleus |
| Synonyms | GLSS; SATB family member 2; SATB homeobox 2; SATB2;Special AT rich sequence binding protein 2. |
| Background | Binds to DNA, at nuclear matrix- or scaffold-associated regions. Thought to recognize the sugar-phosphate structure of double-stranded DNA. Transcription factor controlling nuclear gene expression, by binding to matrix attachment regions (MARs) of DNA and inducing a local chromatin-loop remodeling. Acts as a docking site for several chromatin remodeling enzymes and also by recruiting corepressors (HDACs) or coactivators (HATs) directly to promoters and enhancers. |
| Application Dilution | |
| WB | 1:500-2000 |
| FCM | FCM1:50-100 |
| IF(IHC-P) | IFIHC-P1:100-500 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is SATB2 Recombinant Antibody, APC-Cy7 Conjugated (bsm-62976R-APC-Cy7) validated for, and at what dilution?
bsm-62976R-APC-Cy7 is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(IHC-P)(IF(IHC-P)) (1:100-500); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does SATB2 Recombinant Antibody, APC-Cy7 Conjugated (bsm-62976R-APC-Cy7) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-62976R-APC-Cy7 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise SATB2 Recombinant Antibody, APC-Cy7 Conjugated (bsm-62976R-APC-Cy7)?
KLH conjugated synthetic peptide derived from human SATB2
What are the excitation and emission wavelengths of the APC-Cy7 conjugate (bsm-62976R-APC-Cy7)?
APC-Cy7 on bsm-62976R-APC-Cy7: excitation / emission 650nm/780nm.