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SATB2 Recombinant Antibody, AbBy Fluor-555 Conjugated

Applications

  • WB
  • FCM
  • IF(IHC-P)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-62976r-bf555-100uL
Product Name SATB2 Recombinant Antibody, AbBy Fluor-555 Conjugated
Applications
WB FCM IF(IHC-P) IF(ICC)
Reactivity Human, Mouse, Rat
Specifications
Conjugation AbBy Fluor-555
Host Rabbit
Source KLH conjugated synthetic peptide derived from human SATB2
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 23314
Swiss Prot Q9UPW6
Subcellular location Nucleus
Synonyms GLSS; SATB family member 2; SATB homeobox 2; SATB2;Special AT rich sequence binding protein 2.
Background Binds to DNA, at nuclear matrix- or scaffold-associated regions. Thought to recognize the sugar-phosphate structure of double-stranded DNA. Transcription factor controlling nuclear gene expression, by binding to matrix attachment regions (MARs) of DNA and inducing a local chromatin-loop remodeling. Acts as a docking site for several chromatin remodeling enzymes and also by recruiting corepressors (HDACs) or coactivators (HATs) directly to promoters and enhancers.
Application Dilution
WB 1:500-2000
FCM FCM1:50-100
IF(IHC-P) IFIHC-P1:100-500
IF(ICC) IFICC1:50-200

Frequently asked questions

Which applications is SATB2 Recombinant Antibody, AbBy Fluor-555 Conjugated (bsm-62976R-BF555) validated for, and at what dilution?

bsm-62976R-BF555 is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(IHC-P)(IF(IHC-P)) (1:100-500); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does SATB2 Recombinant Antibody, AbBy Fluor-555 Conjugated (bsm-62976R-BF555) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-62976R-BF555 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise SATB2 Recombinant Antibody, AbBy Fluor-555 Conjugated (bsm-62976R-BF555)?

KLH conjugated synthetic peptide derived from human SATB2

What are the excitation and emission wavelengths of the AbBy Fluor-555 conjugate (bsm-62976R-BF555)?

AbBy Fluor-555 on bsm-62976R-BF555: excitation / emission 553nm/568nm.