Spectrin alpha chain Recombinant Monoclonal Antibody, PE-Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-62985r-pe-cy3-100uL |
| Product Name | Spectrin alpha chain Recombinant Monoclonal Antibody, PE-Cy3 Conjugated |
| Applications |
WB
FCM
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | PE-Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Spectrin alpha chain |
| Clonality | Monoclonal |
| Clone # | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 6708 |
| Swiss Prot | P02549 |
| Subcellular location | Cytoplasm |
| Synonyms | EL2; Elliptocytosis2; erythrocyte; Erythroid alpha spectrin; HPP; HS3; SPH3; SPTA; SPTA1. |
| Background | Spectrin is the major constituent of the cytoskeletal network underlying the erythrocyte plasma membrane. It associates with band 4.1 and actin to form the cytoskeletal superstructure of the erythrocyte plasma membrane. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is Spectrin alpha chain Recombinant Monoclonal Antibody, PE-Cy3 Conjugated (bsm-62985R-PE-Cy3) validated for, and at what dilution?
bsm-62985R-PE-Cy3 is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Spectrin alpha chain Recombinant Monoclonal Antibody, PE-Cy3 Conjugated (bsm-62985R-PE-Cy3) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-62985R-PE-Cy3 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Spectrin alpha chain Recombinant Monoclonal Antibody, PE-Cy3 Conjugated (bsm-62985R-PE-Cy3)?
KLH conjugated synthetic peptide derived from human Spectrin alpha chain
What are the excitation and emission wavelengths of the PE-Cy3 conjugate (bsm-62985R-PE-Cy3)?
PE-Cy3 on bsm-62985R-PE-Cy3: excitation / emission 488nmn/578nm.