Spectrin alpha Recombinant Antibody, PerCP-Cy7 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bsm-62985r-percp-cy7-100uL |
| Product Name | Spectrin alpha Recombinant Antibody, PerCP-Cy7 Conjugated |
| Applications |
WB
FCM
IF(ICC)
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat |
| Specifications | |
| Conjugation | PerCP-Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Spectrin alpha chain |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 6708 |
| Swiss Prot | P02549 |
| Subcellular location | Cytoplasm |
| Synonyms | EL2; Elliptocytosis2; erythrocyte; Erythroid alpha spectrin; HPP; HS3; SPH3; SPTA; SPTA1. |
| Background | Spectrin is the major constituent of the cytoskeletal network underlying the erythrocyte plasma membrane. It associates with band 4.1 and actin to form the cytoskeletal superstructure of the erythrocyte plasma membrane. |
| Application Dilution | |
| WB | 1:500-2000 |
| FCM | FCM1:50-100 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is Spectrin alpha Recombinant Antibody, PerCP-Cy7 Conjugated (bsm-62985R-PerCP-Cy7) validated for, and at what dilution?
bsm-62985R-PerCP-Cy7 is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Spectrin alpha Recombinant Antibody, PerCP-Cy7 Conjugated (bsm-62985R-PerCP-Cy7) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat.
How should bsm-62985R-PerCP-Cy7 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Spectrin alpha Recombinant Antibody, PerCP-Cy7 Conjugated (bsm-62985R-PerCP-Cy7)?
KLH conjugated synthetic peptide derived from human Spectrin alpha chain
What are the excitation and emission wavelengths of the PerCP-Cy7 conjugate (bsm-62985R-PerCP-Cy7)?
PerCP-Cy7 on bsm-62985R-PerCP-Cy7: excitation / emission 482nm/780nm.