TBX21 Recombinant Antibody

$25 ug total protein per lane of various lysates (see on figure) probed with Tbx21 monoclonal antibody, unconjugated (bsm-62988R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with Tbx21 monoclonal antibody, unconjugated (bsm-62988R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

Reactivity

Human

Overview
Catalog #	bsm-62988r
Product Name	TBX21 Recombinant Antibody
Applications	WB FCM
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human TBX21
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	30009
Swiss Prot	Q9UL17
Subcellular location	Nucleus
Synonyms	T bet; T box 21; T PET; TBET; TBLYM; Tbx21; TPET; Transcription factor TBLYM.
Background	Lineage-defining transcription factor which initiates Th1 lineage development from naive Th precursor cells both by activating Th1 genetic programs and by repressing the opposing Th2 and Th17 genetic programs.Activates transcription of a set of genes important for Th1 cell function, including those encoding IFN-gamma and the chemokine receptor CXCR3. Induces permissive chromatin accessibilty and CpG methylation in IFNG.
Application Dilution
WB	1:500-2000
FCM	FCM1:50-100