TRAP1/HSP75 Recombinant Antibody, AbBy Fluor-405 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-62998r-bf405-100uL |
| Product Name | TRAP1/HSP75 Recombinant Antibody, AbBy Fluor-405 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | AbBy Fluor-405 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Hsp75 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 10131 |
| Swiss Prot | Q12931 |
| Subcellular location | Cytoplasm |
| Synonyms | HSP75; HSP90L; Trap1. |
| Background | Chaperone that expresses an ATPase activity. Involved in maintaining mitochondrial function and polarization, downstream of PINK1 and mitochondrial complex I. Is a negative regulator of mitochondrial respiration able to modulate the balance between oxidative phosphorylation and aerobic glycolysis. The impact of TRAP1 on mitochondrial respiration is probably mediated by modulation of mitochondrial SRC and inhibition of SDHA. |
| Application Dilution | |
| WB | 1:500-2000 |
| IF(IHC-P) | IFIHC-P1:50-200 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is TRAP1/HSP75 Recombinant Antibody, AbBy Fluor-405 Conjugated (bsm-62998R-BF405) validated for, and at what dilution?
bsm-62998R-BF405 is validated for WB(WB) (1:500-2000); IF(IHC-P)(IF(IHC-P)) (1:50-200); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does TRAP1/HSP75 Recombinant Antibody, AbBy Fluor-405 Conjugated (bsm-62998R-BF405) react with?
Tested reactivity: Human.
How should bsm-62998R-BF405 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise TRAP1/HSP75 Recombinant Antibody, AbBy Fluor-405 Conjugated (bsm-62998R-BF405)?
KLH conjugated synthetic peptide derived from human Hsp75
What are the excitation and emission wavelengths of the AbBy Fluor-405 conjugate (bsm-62998R-BF405)?
AbBy Fluor-405 on bsm-62998R-BF405: excitation / emission 401nm/421nm.