TRAP1/HSP75 Recombinant Antibody, PerCP Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-62998r-percp-100uL |
| Product Name | TRAP1/HSP75 Recombinant Antibody, PerCP Conjugated |
| Applications |
WB
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | PerCP |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Hsp75 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 10131 |
| Swiss Prot | Q12931 |
| Subcellular location | Cytoplasm |
| Synonyms | HSP75; HSP90L; Trap1. |
| Background | Chaperone that expresses an ATPase activity. Involved in maintaining mitochondrial function and polarization, downstream of PINK1 and mitochondrial complex I. Is a negative regulator of mitochondrial respiration able to modulate the balance between oxidative phosphorylation and aerobic glycolysis. The impact of TRAP1 on mitochondrial respiration is probably mediated by modulation of mitochondrial SRC and inhibition of SDHA. |
| Application Dilution | |
| WB | 1:500-2000 |
| IF(IHC-P) | IFIHC-P1:50-200 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is TRAP1/HSP75 Recombinant Antibody, PerCP Conjugated (bsm-62998R-PerCP) validated for, and at what dilution?
bsm-62998R-PerCP is validated for WB(WB) (1:500-2000); IF(IHC-P)(IF(IHC-P)) (1:50-200); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does TRAP1/HSP75 Recombinant Antibody, PerCP Conjugated (bsm-62998R-PerCP) react with?
Tested reactivity: Human.
How should bsm-62998R-PerCP be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise TRAP1/HSP75 Recombinant Antibody, PerCP Conjugated (bsm-62998R-PerCP)?
KLH conjugated synthetic peptide derived from human Hsp75
What are the excitation and emission wavelengths of the PerCP conjugate (bsm-62998R-PerCP)?
PerCP on bsm-62998R-PerCP: excitation / emission 482nm/677nm.