Phospho-TRIM28 (Ser824) Recombinant Antibody, AbBy Fluor-647 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-63001r-bf647-100uL |
| Product Name | Phospho-TRIM28 (Ser824) Recombinant Antibody, AbBy Fluor-647 Conjugated |
| Applications |
WB
|
| Reactivity | Human |
| Specifications | |
| Conjugation | AbBy Fluor-647 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human TIF1 beta around the phosphorylation site of S824 |
| Modification Site | Ser824 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 10155 |
| Swiss Prot | Q13263 |
| Subcellular location | Nucleus |
| Synonyms | KAP 1; KRAB associated protein 1; KRIP1; RNF96; TF1B; TIF1 beta; TIF1B; Trim28; Tripartite motif containing 28. |
| Background | Nuclear corepressor for KRAB domain-containing zinc finger proteins (KRAB-ZFPs). Mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 (which specifically methylates histone H3 at 'Lys-9' (H3K9me)) to the promoter regions of KRAB target genes. Enhances transcriptional repression by coordinating the increase in H3K9me, the decrease in histone H3 'Lys-9 and 'Lys-14' acetylation (H3K9ac and H3K14ac, respectively) and the disposition of HP1 proteins to silence gene expression. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is Phospho-TRIM28 (Ser824) Recombinant Antibody, AbBy Fluor-647 Conjugated (bsm-63001R-BF647) validated for, and at what dilution?
bsm-63001R-BF647 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-TRIM28 (Ser824) Recombinant Antibody, AbBy Fluor-647 Conjugated (bsm-63001R-BF647) react with?
Tested reactivity: Human.
How should bsm-63001R-BF647 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-TRIM28 (Ser824) Recombinant Antibody, AbBy Fluor-647 Conjugated (bsm-63001R-BF647)?
KLH conjugated synthetic peptide derived from human TIF1 beta around the phosphorylation site of S824
What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bsm-63001R-BF647)?
AbBy Fluor-647 on bsm-63001R-BF647: excitation / emission 650nm/665nm.