Thioredoxin Recombinant Antibody

$25 ug total protein per lane of various lysates (see on figure) probed with Thioredoxin monoclonal antibody, unconjugated (bsm-63002R) at 1:2000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with Thioredoxin monoclonal antibody, unconjugated (bsm-63002R) at 1:2000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-63002r
Product Name	Thioredoxin Recombinant Antibody
Applications	WB FCM
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Thioredoxin
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	7295
Swiss Prot	P10599
Subcellular location	Cytoplasm, Nucleus, Secreted
Synonyms	TXN; ADF; ATL-derived factor; SASP; Thioredoxin; TRX1; Thioredoxin delta 3; TRX; TXN delta 3; TRDX.
Background	Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity.
Application Dilution
WB	1:500-2000
FCM	FCM1:50-100