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Thioredoxin Recombinant Antibody, APC Conjugated

Applications

  • WB
  • FCM

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-63002r-apc-100uL
Product Name Thioredoxin Recombinant Antibody, APC Conjugated
Applications
WB FCM
Reactivity Human, Mouse, Rat
Specifications
Conjugation APC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Thioredoxin
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 7295
Swiss Prot P10599
Subcellular location Cytoplasm, Nucleus, Secreted
Synonyms TXN; ADF; ATL-derived factor; SASP; Thioredoxin; TRX1; Thioredoxin delta 3; TRX; TXN delta 3; TRDX.
Background Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity.
Application Dilution
WB 1:500-2000
FCM FCM1:50-100

Frequently asked questions

Which applications is Thioredoxin Recombinant Antibody, APC Conjugated (bsm-63002R-APC) validated for, and at what dilution?

bsm-63002R-APC is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Thioredoxin Recombinant Antibody, APC Conjugated (bsm-63002R-APC) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-63002R-APC be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Thioredoxin Recombinant Antibody, APC Conjugated (bsm-63002R-APC)?

KLH conjugated synthetic peptide derived from human Thioredoxin

What are the excitation and emission wavelengths of the APC conjugate (bsm-63002R-APC)?

APC on bsm-63002R-APC: excitation / emission 650nm/660nm.