Thioredoxin Recombinant Antibody, Cy5.5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-63003r-Cy5.5-100uL |
| Product Name | Thioredoxin Recombinant Antibody, Cy5.5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Thioredoxin |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 7295 |
| Swiss Prot | P10599 |
| Subcellular location | Cytoplasm, Nucleus, Secreted |
| Synonyms | TXN; ADF; ATL-derived factor; SASP; Thioredoxin; TRX1; Thioredoxin delta 3; TRX; TXN delta 3; TRDX. |
| Background | Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. |
| Application Dilution | |
| WB | 1:500-2000 |
| IF(IHC-P) | IFIHC-P1:50-200 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is Thioredoxin Recombinant Antibody, Cy5.5 Conjugated (BSM-63003R-CY5-5) validated for, and at what dilution?
BSM-63003R-CY5-5 is validated for WB(WB) (1:500-2000); IF(IHC-P)(IF(IHC-P)) (1:50-200); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Thioredoxin Recombinant Antibody, Cy5.5 Conjugated (BSM-63003R-CY5-5) react with?
Tested reactivity: Human.
How should BSM-63003R-CY5-5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Thioredoxin Recombinant Antibody, Cy5.5 Conjugated (BSM-63003R-CY5-5)?
KLH conjugated synthetic peptide derived from human Thioredoxin
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BSM-63003R-CY5-5)?
Cy5.5 on BSM-63003R-CY5-5: excitation / emission 675nm/694nm.