UBA1 Recombinant Antibody, Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-63004r-cy3 |
| Product Name | UBA1 Recombinant Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human UBA1 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 7317 |
| Swiss Prot | P22314 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | A1S9; A1S9T; A1ST; AMCX1; CFAP124; GXP1; POC20; SMAX2; Uba1; UBA1A; UBE1; UBE1X. |
| Background | Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thioester and free AMP. |
| Application Dilution | |
| WB | 1:500-2000 |
| IF(IHC-P) | IFIHC-P1:50-200 |
Frequently asked questions
Which applications is UBA1 Recombinant Antibody, Cy3 Conjugated (bsm-63004R-Cy3) validated for, and at what dilution?
bsm-63004R-Cy3 is validated for WB(WB) (1:500-2000); IF(IHC-P)(IF(IHC-P)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does UBA1 Recombinant Antibody, Cy3 Conjugated (bsm-63004R-Cy3) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-63004R-Cy3 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise UBA1 Recombinant Antibody, Cy3 Conjugated (bsm-63004R-Cy3)?
KLH conjugated synthetic peptide derived from human UBA1
What are the excitation and emission wavelengths of the Cy3 conjugate (bsm-63004R-Cy3)?
Cy3 on bsm-63004R-Cy3: excitation / emission 512,550nm/570,615nm.