K27-linkage specific ubiquitin Recombinant Antibody, AbBy Fluor-405 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-63007r-bf405 |
| Product Name | K27-linkage specific ubiquitin Recombinant Antibody, AbBy Fluor-405 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-405 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human K27-linkage specific ubiquitin |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 7314 |
| Swiss Prot | P0CG47,P0CG48 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | FLJ25987; MGC8385; ubiquitin B; Ubiquitin; UBCEP1; UBCEP2; RPS27A. |
| Background | Ubiquitin: Exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). |
| Application Dilution | |
| WB | 1:1000-2000 |
| IF(IHC-P) | IFIHC-P1:100-500 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is K27-linkage specific ubiquitin Recombinant Antibody, AbBy Fluor-405 Conjugated (bsm-63007R-BF405) validated for, and at what dilution?
bsm-63007R-BF405 is validated for WB(WB) (1:1000-2000); IF(IHC-P)(IF(IHC-P)) (1:100-500); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does K27-linkage specific ubiquitin Recombinant Antibody, AbBy Fluor-405 Conjugated (bsm-63007R-BF405) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-63007R-BF405 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise K27-linkage specific ubiquitin Recombinant Antibody, AbBy Fluor-405 Conjugated (bsm-63007R-BF405)?
KLH conjugated synthetic peptide derived from human K27-linkage specific ubiquitin
What are the excitation and emission wavelengths of the AbBy Fluor-405 conjugate (bsm-63007R-BF405)?
AbBy Fluor-405 on bsm-63007R-BF405: excitation / emission 401nm/421nm.